Gel Permeation Chromatography - Size Separation Chromatography (GPC - SEC)

Brand / Model: Malvern / Viscotek 270max

It is a chromatography technique that is particularly applicable to high molecular mass species. It is based on different diffusion and permeation rates as different molecules pass through a porous medium. The fillers are composed of small size (~ 10 mm) silica or polymer compounds containing a smooth pore network into which solute and solvent molecules can diffuse. Molecules in the pores of the fillers are effectively captured and removed by the mobile phase. The duration of its residence in the pores depends on the size of the molecule. While large molecular weight compounds exit the column more quickly, smaller molecules remain in the pores more so they are eluted last.

Technicial Specifications

Column Oven Temperature Range: 10-100 ^oC

Light Scattering Detector

Light source: Diode capable of temperature control, 3 mW, 670 nm Cell: 18 µL

Refractive Index detector

Light Source: Tungsten lamp

Cell: 450 quartz glass, 9 µL volume

Temperature control: 1 ^oC step range from 35 ^oC to 55 ^oC

Uv detector:

Wavelength: 190-700 nm

Light source: Deuterium Cell: 10 µL

Scope of application

It is used to determine the Mn, Mw / Mn values of the polymers.